LaVision BioTec Ultramicroscope


 

Download our operating protocol.

Technical Details:

LaVision BioTec Ultramicroscope fluorescence light sheet imaging for 3D image reconstruction in large samples . Cleared wholemount specimens are illuminated using a thin light sheet and imaged on a high resolution sCMOS camera orthogonal to illumination. Selective illumination ensures decreased photobleaching. System is equipped with dual lightsheet illumination.

 

Microscope:
Based around an Olympus MVX10 zoom microscope body (variable zoom 0.63 - 6.3 X)

 

Light sheet excitation lines:
OPSL (50mW), 488nm.
OPSL (50mW), 561nm.
Diode laser (50mW), 647nm
Diode laser (50mW), 785nm

 

Camera:
Andor Neo sCMOS camera: scientific low noise CMOS camera.

 

Detection Channels:

Example-Color

Filter

FITC

525/50

Texas Red

595/40

TRIC

620/60

Cy5

680/30

ICG

840/50

 

 

Clearing and Sample Preparation: