IVIS Lumina II


Download our operating protocol.

 

Technical Details:

 

System: Lumina II
Small animal, in vivo, quantitative, fluorescence and bioluminescence, molecular imaging system.

 

Software: Living Image v 4.1 

 

Excitation wavelengths: high resolution 35 nm bandwidth centered at: 430, 465, 500, 535, 570, 605, 640, 675, 710, 745.

 

Standard filter set and fluorophores:

Label

Excitation bandpass (nm)

Emission bandpass (nm)

background bandpass (nm)

Fluorophores

Green

445-490

515-575

410-440

GFP, EGFP, FITC

Red

500-550

575-650

460-490

DsRed2-1, 605 Qdot Bioconjugate

Far Red

615-665

695-770

580-610

Cy5.5, Alexa 660, Alexa 680, 705 QDot Bioconjugate

NIR

710-760

810-875

665-695

Indocyanine green (ICG), 805 Qdot Bioconjugate

 

 

CCD Camera: Andor Technologies scientific grade, back-thinned, back-illuminated, large format CCD.

CCD Camera

Specification

Sensor type

Back illuminated

CCD Format

1024 X 1024

Pixel Dimensions (microns)

13 X 13

Quantum Efficiency

>85% 400-700

Readout noise

<= 2 e RMS

Dark Charge

<0.0015 e/pix/sec

CCD Digitization

16 bit

Cooling

Thermoelectric

Capacity: Up to 5 mice or 2 Rats

 

Gas anesthesia system: The isoflurane anesthesia system allows for the anesthesia of up to five mice in the imaging chamber during imaging. There is a separate chamber for inducing anesthesia prior to imaging.

 

Accessories:

Emission filter options:

label

Emission bandpass 20nm, centered at (nm):

Fluorophores

500 Series

500,520,540,560, 620.

GFP, YFP and PKH26

700 Series

720, 740, 760, 780, 800, 840.

ICG and Xenofluor 750

 

Lens options: XFOV-24 lens attachment for expanded field of view (5 mice).