Zeiss AxioObserver.Z1 Inverted Microscope with ApoTome Optical Sectioning and a Live Cell Imaging System


 

Dowlnoad our operating protocol.

 

 

Technical Details:

 

Microscope frame: Zeiss AxioObserver.Z1 motorized inverted epifluorescence microscope.

Illumination with metal halide HXP-120 Lamp and Colibri high performance LED at 365nm, 470nm, 555nm and 625nm.

 

Objectives:

 Objective Type

Magn.

Contrast

Numerical Aperture

Working Distance (mm)

Immersion

EC Plan-Neofluar

5

-

0.16

18.5

air

Plan-Apochromat

10

DIC

0.45

2.0

air

N-Achroplan

10

PH1

0.25

6.0

air

Plan-Apochromat

20

DIC

0.8

0.55

air

LD Plan-Neofluor Corr*

20

PH2

0.4

7.9

air

Plan-Apochromat
Corr

40

DIC

0.95

0.25

air

LD C-Apochromat
Corr*

40

DIC

1.1

0.62

water

Plan-Apochromat

63

DIC

1.4

0.19

oil

 *Available on request

 

 Filters:

Filter Set

Excitation

Dichroic

Emission

Fluorophores

Zeiss filter set 49

G365

395

BP 445/50

DAPI

Zeiss filter set 38

BP 470/40

495

BP 525/50

FITC, Alexa 488, EGFP

Custom Filter Set

BP 377/50

409

BP 525/50

eCFP, BV510

AHF F56-429

DBP
468/40 & 553/30

493 & 574

DBP 512/30 & 630/100

GFP & DsRed

upon request

Zeiss filter set 43

BP 545/25

570

BP 605/70

TRITC, Cy3, Alexa 546, DsRED

Zeiss filter set 50

BP 640/30

660

BP 690/50

Cy5, Alexa 647

 

monochrome Camera: Orca flash 16 bit data depth 4.2 Megapixel monochrome sCMOS camera.

color Camera: Axiocam 506 color 6 Megapixel 12x10mm Chipsize

 

Software: ZEN Blue 2012

 

Live Cell Imaging Accessories: Pecon Large Chamber Incubation System S, comprising:

  • Incubator and heating unit XL multi S1 (large heated chamber around inverted microscope)
  • Temp module S.
  • CO2 module S.
  • Heated insert PS1 with CO2 cover PMS1.
  • Heated insert P Lab-Tek with CO2 cover.

 

Zeiss ApoTome:
The Zeiss ApoTome uses structured illumination giving wide field optical sectioning to remove blur arising from out of focus fluorescence signal. The Zeiss Campus webpages show the ApoTome principle and operation.