BD FACS Aria III


 

Download our operating protocol.

 

Technical Details:

The BD FACS Aria III high speed cell sorter.
Technical details.

Type:
5 Laser 5-2-3-3 with aerosole management system.

Lasers:
561nm solid state, 488nm solid state, 633nm HENE, and 405nm, or 365 Diode laser.

Detectors:
Forward scatter.
Laser 1: Octagon with 3 fluorescence detectors
Laser 2: Trigon with 3 fluorescence detectors and side scatter
Laser 3: Trigon with 3 fluorescence detectors
Laser 4: Octagon with 5 fluorescence detectors

Software: BD Diva

Filtersettings:

 Laser

Position

LP

Filter

Common dyes

405/365nm Octragon

A

 600 

 610/20 

QDot 605

violett laser

B

 502

 510/50

AmCyan; HorizonV500; Alexa 430

C

 450/40

Dapi; Hoechst; Pacific Blue; AF405

633nm Trigon

A

 755

 780/60

APC-Cy7

red laser

B

 690

 730/45

Alexa700

C

 660/20

TOPRO3; Alexa674; APC

488nm Trigon

A

 655

 695/40

PerCP5.5; PI; PeCy5; PerCP

blue laser

B

 502

 530/30

FITC; GFP; AF488

C

 488/10

(SSC)

561nm Octagon

A

 735

 780/60

PeCy7

yellow/green laser

B

 685

 710/50

PeCy5.5

C

 630

 670/14

PeCy5

D

 600

 610/20

mCherry; PeTexasRed; PI; Alexa 568

E

 582/15

PE; Alexa555; Cy3; RFP

 

Sort collection devices:

Room temperature

2-way

12x75mm

15ml tubes

4-way

12x75mm

1,5ml tubes

Adjustable temperature (5°C-40°C)

2-way

15ml tubes

4-way

12x75mm tubes

1,5ml tubes