Olympus BX51 Upright Fluorescence Microscope


Download our operating protocol.

 

Technical Details:

Microscope Frame: Olympus BX51 manual upright epifluorescence microscope, with 100W mercury Lamp.

Objectives:

Objective Type

Magnification

Contrast

Numerical Aperture

Working Distance (mm)

Immersion

PlanApo N

1.25

Bright field

0.04

5.1

air

UPlanFL N

4

Bright field

0.13

17

air

UPlanFL N

10

Bright field

0.3

10

air

UPlanSApo

20

Bright field

0.75

0.6

air

UplanSApo

60

Bright field

1.35

0.15

oil

 

 Filters:

Filter Set

Excitation

Dichroic

Emission

Fluorophores

DAPI

360-370

400

460/50

DAPI

NB

470-490

515

520 LP

FITC

FITC

470/40

495

525/50

eGFP, FITC, Alexa 488

TxRED

560/55

595

645/75

Texas Red,TRITC, Cy3, Alexa 546, DsRED

Cy5*

628/40

660

692/40

Cy5*

 * Please be aware, that the excitation of this Fluorochrome is very week. Make sure you only stain highly expressed structures in your sample.

 

Camera: Olympus DP70 1.5 Megapixel colour ccd camera.

 

Software: Cell sens Dimension