Zeiss ELYRA PS.1 Super-resolution Microscope with Structured Illumination (SIM), Pal-M/STORM localization and TIRF, combined with an LSM710 Confocal


 

 

Download our operating protocol.

 

Technical Details:

 

Microscope frame: Zeiss AxioObserver.Z1 motorized inverted epifluorescence microscope, with a X-Cite 120 Widefield fluorescence observation system filterset:

Filterset 25 HE

Excitation:

Beam splitter:

Emission:

TBP 405 + 495 + 575 (HE)

TFT 435 + 510 + 600 (HE)

TBP 460 + 530 + 625 (HE)

with a definite focus incl. a motorized objective revolver ACR; a scanningstage 130x100 DC

Kondensormodule DIC II/0.55 and DIC III /0.55 with Polarisor available

 

Objectives:

Objective Type

Magn.

Contrast

Numerical Aperture

Working Distance (mm)

Immersion

Plan-Apochromat

20

DIC

0.8

0.55

air

Plan-Apochromat

63

DIC

1.4

0.19

oil

C-Apochromat
Corr

63

DIC

1.2

0.28

water

αlpha Plan-Apochromat

100

DIC

1.46

0.11

oil

 

 

Lasers and Filters:

LSM 710 confocal

Superresolution Pal-M/STORM; SIM

 

3 spectral detection chanels 6 laserlines

- 561nm Laser (20mW)

- 458,488,514 nm Argon Multiline (25mW)

- 405nm Diode Laser (30mW)

-633nm HeNe Laser

 

 

-405nm Diode Laser 50mV

-488nm OPSL Laser 200mV

-561nm OPSL Laser 200mV

-642nm Diode Laser 150mV

Lasers

spectral detection with 3 channel

 

MBS-405 BP420-480/LP750

MBS-488 BP495-575/LP750

MBS-561 BP570-650/LP750

MBS-642 LP655

 

Filters

 

 

 

Camera:

  • Andor EM CCD Camera iXon DU 897 with 512x512 active Pixels and 16 x 16 µm Pixel size
  • PCO Edge sCMOS Camera

 

 

 

Software:

ZEN system 2012 (black edition)

 

Live Cell Imaging Accessories: Pecon Large Chamber Incubation System S, comprising:

  • Incubator and heating unit XL multi S1 (large heated chamber around inverted microscope)
  • Temp module S1.
  • CO2 module S1.
  • Heated insert PS1 with CO2 Modul and cover PMS1.

 

Elyra specification data sheets:

- product sheet

- enter the world of superresolution

- white paper